Analytical ultracentrifugation reveals a linear relationship of the Stokes radius on the expected molar mass for the different oligomeric forms of VASP in a log–log plot. Logarithm of the Stokes radii (circles) as calculated from the sedimentation coefficients from Fig. 1B plotted against the expected molar masses calculated from amino acid composition of the different oligomeric forms of VASP (VASP-1M, 77.7 kg/mol; VASP-2M, 163.9 kg/mol; VASP-3M, 246.4 kg/mol; VASP-4M, 332.8 kg/mol; and VASP-6M, 489.5 kg/mol). Linear regression (solid red line) resulted in a slope m = 0.474, an intercept b = −0.226, and a correlation coefficient R2 = 0.993.