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Pelleting assay showing stoichiometric binding of PdBtubC to PdBtubAB filaments. Replicate experiment to the one shown in Fig. 1D. Fourfold excess of BtubC to BtubAB (molar, C to AB heterodimer) leads to roughly the same amount of BtubC in the pellet compared with equimolar quantities. Excess BtubC protein remains in the supernatant and does not spin down, indicating a specific interaction of BtubC with BtubAB heterodimers in the filaments.
