Table S1.
Crystallography and cryoEM data
| Sample | Prosthecobacter vanneervenii PvBtubC EMTS derivative | Prosthecobacter vanneervenii PvBtubC native | Prosthecobacter dejongeii PdBtubABC |
| NCBI database ID | CAP16140.1 | CAP16140.1 | AA012155.1, AA012159.2, this work |
| Constructs | 1–256-KLHHHHHH | 1–256-KLHHHHHH | A: 1–473, no tag, B: 1–426 no tag, C: MGSSHHHHHH-SSGLLPRGSH-1–257 |
| Method | Crystallography SAD | Crystallography | CryoEM, |
| 0.5 mM EMTS | helical reconstruction | ||
| Data collection | |||
| Beamline/microscope | ESRF ID30B | ESRF ID29 | FEI Polara G2, Falcon III |
| Wavelength/energy | 1.006 Å | 0.977170 | 300 kV |
| Crystal/helical | |||
| Space/point group | H32 | H32 | |
| Cell, Å | 120.0, 120.0, 239.1 | 119.7, 119.7, 239.4 | |
| Twist/rise | −5.54°, 79.31 Å | ||
| Data | |||
| Resolution, Å | 2.8 | 2.5 | 3.6 |
| Completeness*, % | 100 (100) | 99.4 (100) | |
| Multiplicity* | 11.8 (11.3) | 4.9 (5.2) | |
| (I)/σ(I)* | 17.8 (2.4) | 10.8 (2.7) | |
| Rmerge* | 0.102 (1.036) | 0.108 (0.674) | |
| Rpim* | 0.044 (0.467) | 0.054 (0.330) | |
| CC1/2 | 0.999 (0.726) | 0.996 (0.690) | |
| Anomalous correlation | 0.24 (0.0) | ||
| Mercury sites | 4 | ||
| Images, pixel size | 6105, 1.34 Å | ||
| Defocus range, dose | −1.5 to −3 µm, 40 e/Å2 | ||
| Helical segments | 257,661 | ||
| Refinement | |||
| R/Rfree† | 0.186/0.237 | 0.32 | |
| Models | 2 chains/ASU: | 1 BtubAB heterodimer: A residues 3–435, B 2–37; 46–273;281–426 | |
| Residues 2–255, | 0.014 | ||
| 92 waters | |||
| Bond length rmsd, Å | 0.015 | ||
| Bond angle rmsd, ° | 1.716 | 1.556 | |
| Favored‡, % | 100 | 99.5 | |
| Disallowed‡, % | 0 | 0 | |
| MOLPROBITY score | 95th percentile | 100th percentile | |
| PDB/EMDB IDs | 5O01 | 5O09, EMD-3726 |
*
Values in parentheses refer to the highest recorded resolution shell.
†
Five percent of reflections were randomly selected before refinement.
‡
Percentage of residues in the Ramachandran plot (PROCHECK “most favored” and “additionally allowed” added together).