Fig. 4.

Membrane partitioning is essential for cytokinesis. (A) Time-lapse images of a representative BFA-treated cell attempting cytokinesis. Note that furrow ingression consistently stops shortly after the start of cytokinesis (Movie S5). (Scale bar: 5 μm.) (B) Histogram of cytokinesis timing for BFA-treated (25 µM, n = 83) and control cells (0.35% DMSO, n = 106). Data for BFA acquired from three independent movies, data for DMSO control from two independent movies. (C) Functional box plot of bootstrapped LOESS curves for furrow ingression measurements of BFA-treated (pink, n = 12) and control cells (blue, n = 12).