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. 2017 Jul 3;114(29):E5940–E5949. doi: 10.1073/pnas.1708065114

Fig. 5.

Fig. 5.

The molecular mechanism behind the recruitment of BLM and BRCA1 to ALT telomeres. (A–C) BRCA1 localized to ALT telomeres. U2-OS cells (A and B) or Saos-2 cells (C) were transfected with siRNA targeting either Luciferase control (Luc) or two different siRNA targeting FANCM (M and MU). Cells were then costained with an antibody recognizing BRCA1 together with a PNA probe recognizing the telomere (A and C, TelC) or an antibody recognizing TRF1 (B). (D) U2-OS cells were transfected with siRNA targeting Luciferase (Luc), BLM (BLM1), FANCM (MU), or both BLM and FANCM (BLM1+MU). Cells were then transfected with either GFP or GFP-BLM that is resistant to the BLM1 siRNA and costained with antibodies recognizing either BRCA1 or TRF1. (E) U2-OS cells were transfected with siRNA targeting Luciferase (Luc), BRCA1 (BU), FANCM (MU), or both BRCA1 and FANCM (BU+MU). Cells were then transfected with either Vector or BRCA1 ORF and costained with antibodies recognizing either BLM or TRF1. (F–H) U2-OS cells were transfected with different siRNAs and then costained with antibodies recognizing TRF1, BRCA1 (F and G), or BLM (H). More than 200 cells were counted for each sample. All error bars are SD and obtained from two independent experiments. Standard two-sided t test: *P < 0.05, **P < 0.01, ***P < 0.001.