Fig. S2.

FANCM deficiency induces replication stress in Saos-2 and HuO9 cells. Saos-2 (A–F) or HuO9 (G–J) cells were transfected with siRNA either targeting Luciferase (Luc) or two different siRNA targeting FANCM (FANCM and FANCM-U). Cells were then costained with either a PNA probe recognizing the G-rich strand of telomeres (TelC) or different antibodies as indicated on the top in A, B, D, E, G, and H. All nuclei were also stained with DAPI. More than 200 cells were counted for each sample. All error bars are SD obtained from two different experiments. Standard two-sided t test: **P < 0.01, ***P < 0.001.