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. Author manuscript; available in PMC: 2018 Jul 28.
Published in final edited form as: Cancer Lett. 2017 Apr 12;399:29–43. doi: 10.1016/j.canlet.2017.04.005

Figure 5. Overexpression of IFITM1 promoted breast cancer cell proliferation and invasion in vivo.

Figure 5

(a) Intact female NSG mice were orthotopically injected with MCF-7/C9Con and MCF-7/IF-2 cells and tumors measured by digital calipers weekly. Tumor volume (mm3) was calculated and charted over time. (b) The tumors were excised and imaged at day 56 and final tumor volume (mm3) determined. (c) IFITM1 and Ki67 expression was determined by immunohistochemical staining. The percent of Ki67 positive cells was determined by counting four separate 40X fields (lower panel). (d) Female NSG mice were injected with MCF-7/C9Con and MCF-7/IF-2 cells using the MIND model. Smooth muscle actin (SMA) staining by immunohistochemistry elucidated the milk duct. Arrow heads indicate invasive lesions which were quantified per duct in a 10X field. Numbers represent averages of 5 fields in 3 different mammary glands for each group (lower panel). *p < 0.05, ** p< 0.01