Skip to main content
. Author manuscript; available in PMC: 2018 Jul 28.
Published in final edited form as: Cancer Lett. 2017 Apr 12;399:29–43. doi: 10.1016/j.canlet.2017.04.005

Figure 9. Loss of IFITM1 induces p21 nuclear translocation.

Figure 9

(a) Fresh whole cell lysates from 80% confluent MCF-7, and MCF-7:5C were separated into nuclear (laminin B) and cytoplasmic (β-actin) fractions. Protein expression of p21 and IFITM1 was determined by immunoblotting. (b) MCF-7:5C/shIF cells with and without doxycycline (± Dox) exposure were also treated with 10μM ruxolitinib (Rux). After 48 hours Rux treatment, cells were harvested and fresh whole cell lysates were fractionated and immunoblotted for p21 and IFITM1 protein expression. B-actin and Lamin B expression indicate the purity of the cytoplasmic and nuclear fractions respectively. (c) MCF-7:5C/shIF cells with and without doxycycline (± Dox) exposure were also treated with 10μM ruxolitinib (Rux). After 48 hours Rux treatment, p21 and IFITM1expression and localization as determined by immunofluorescent staining.