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. Author manuscript; available in PMC: 2017 Jul 27.
Published in final edited form as: J Am Chem Soc. 2016 Mar 9;138(11):3844–3855. doi: 10.1021/jacs.6b00162

Figure 2.

Figure 2

Validation of the foci-based screening assay using known DSB repair inhibitors. (A) Percent of cells with ≥15 γH2AX (left) or 53BP1 (right) foci as a function of time after exposure to 10 Gy IR. (B) Effects of BEZ-235 (left) and NU7441 (right) on resolution of γH2AX and 53BP1 foci. Cells were pretreated with BEZ-235 (0.025–2.8 µM) or NU7441 (0.005–5 µM) for 1 h and then subjected to 10 Gy irradiation. The percentages of cells with ≥15 foci are shown as functions of time postirradiation. (C, D) Impaired foci resolution in cells treated with DNA repair inhibitors. Shown are (C) immunofluorescence images and (D) percentages of cells with ≥15 53BP1 or γH2AX foci in cell populations pretreated with 5 µM NU7441 or 1 µM BEZ-235 for 1 h, exposed to 10 Gy IR, and analyzed 24 h postirradiation.