Figure 7.

Reverse-phase protein array analysis in MK-2206–treated DOHH2 and LP cell lines. A: Supervised hierarchical clustering heatmap of 285 proteins analyzed in DOHH2 and LP cell lines. For each heatmap, the top three rows are triplicate controls, and the bottom six rows are MK-2206 (IC₇₅) treatment for 24 and 48 hours, respectively. B: Heatmap for significantly up-regulated and down-regulated proteins in DOHH2 and LP cell lines after MK-2206 treatment. Red and green bars indicate up-regulation and down-regulation, respectively. C: Schematic illustration of the alterations of AKT signaling pathways after MK-2206 treatment. Detailed descriptions are in the main text. Green arrows and blunted lines indicate tumor-suppressing effects; red arrows and blunted lines indicate tumor-promoting effects. D: Examples of down-regulated phosphorylated AKT (pAKT) and AKT target proteins significant in both DOHH2 and LP cell lines after MK-2206 treatment. ABC, activated B-cell–like; GCB, germinal center B-cell–like; PTK, protein tyrosine kinase.