. 2017 Aug;187(8):1700–1716. doi: 10.1016/j.ajpath.2017.04.009

Table 5.

Up- and Down-Regulated Proteins after MK-2206 Treatment in DOHH2 and LP Cell Lines

Functional categories	In DOHH2 (GCB-DLBCL)		In LP (ABC-DLBCL)
Functional categories	Increased	Decreased	Increased	Decreased
Cell cycle, checkpoint, DNA damage response, DNA replication, gene expression	Histone-H3, eEF2K, p27-Kip-1,^∗ p16INK4a, 53BP1, p21,^∗^† Cyclin-E1,^† ATM,^† PARP-cleaved^†	Chk1, Rb_pS807_S811, PLK1, Cyclin-B1,^∗ FoxM1,^∗ Rad51, Chk1_pS296, PCNA, Rb, ATR, UBAC1, GCN5L2, CDK1, MSH2, MSH6, Chk1_pS345, CDC2-p34, Chk2, Aurora-B	Pdcd4,^∗ Cyclin-E1, PARP-cleaved^†	Cyclin-B1,^∗ PLK1, Rb_pS807_S811, FoxM1,^∗ Chk1, Aurora-B, MSH2, ATR, CDC2-p34, Rb, MSH6, UBAC1, CDK1, PCNA, Chk1_pS296, ADAR1, ARID1A, Cyclin-D1^∗
Apoptosis, autophagy	Bcl-2, Mcl-1, Puma, Bim,^∗ Bad_pS112,^∗ Caspase-7-cleaved,^∗ Caspase-3,^∗ Bax,^∗ Beclin,^∗ Caspase-9-cleaved,^∗ FoxO3a^∗	FoxO3a_pS318_S321,^∗ p53, Bid, XIAP^∗	Mcl-1, Bim,^∗ Puma, Bcl-2, Caspase-7,^∗ Smac, Bak, p53, Bad,^∗ Caspase-9^∗	Bcl2A1
Signaling, cell growth, immune response	S6_pS235_S236,^∗ p38_pT180_Y182, MEK1_pS217_S221, NF-kB-p65_pS536, Rictor,^∗ PAR, PDGFR-β,^∗ MAPK_pT202_Y204, Lck, PKC-α, PI3K-p110-α-R,^∗ PI3K-p85,^∗ Tuberin (TSC2),^∗ PTEN,^∗ Axl,^∗ Caveolin-1, PREX1, PKC-β-II_pS660, PKC-α_pS657,^† FAK_pY397,^∗^†IGFBP5,^∗^† SHP-2_pY542,^∗^† Gab2,^∗^† Akt^∗^†	Akt_pS473,^∗ Akt_pT308,^∗ eIF4G,^∗ TTF1, 4E-BP1_pS65,^∗ FAK, mTOR,^∗ MEK1, c-Myc,^∗ HER3_pY1289,^∗ c-Met,^∗ S6_pS240_S244^∗^†	PAR, Lck, c-Myc,^∗ YAP_pS127,^∗ Notch1, Pdcd-1L1,^∗ PKC-α_pS657, Axl,^∗ Caveolin-1, PDGFR-β,^∗ Notch3, NF-κB-p65_pS536, PKC-δ_pS664, PKC-β-II_pS660	Akt_pS473,^∗ eIF4G,^∗ p70-S6K_pT389,^∗ MEK1, 4E-BP1_pS65,^∗ PRAS40_pT246,^∗ C-Raf, mTOR^∗
Cell adhesion, gap junction, extracellular matrix, exocytosis, endocytosis, angiogenesis	Fibronectin, Connexin-43, Annexin-I, Annexin-VII, Claudin-7,^† CD44-M^†	TFRC, VEGFR-2^∗	Connexin-43, E-Cadherin,^∗ Claudin-7, Annexin-VII, CD44, Annexin-I^†
Metabolism, hypoxia	LDHA^†	Hif-1α,^∗ GSK-3b_pS9,^∗ GSK-3ab_pS21_S9,^∗ Hexokinase-II,^∗ ACC1, ACC_pS79, GSK-3ab^∗	G6PD, ATP5A	GSK-3b_pS9,^∗ GSK-3ab_pS21_S9,^∗ SDHA, Gys, Hexokinase-II,^∗ GSK-3ab^∗
Protein folding	HSP27_pS82, Cyclophilin, HSP27	HSP70	HSP27

The order of proteins is according to the fold-change at 24 hours after treatment.

ABC, activated B-cell–like; DLBCL, diffuse large B-cell lymphoma; GCB, germinal center B-cell–like.

^∗

Proteins upstream or downstream of AKT signaling and Pdcd-1L1.

^†

Proteins that showed increase only at 48 hours.