Fig. 4.

a Transwell chemotaxis migration assay (n = 3). Error bars represent SEM, and statistical significance was determined by two-tailed paired t test; ** p < 0.0025, * p < 0.01. Original magnification × 200, scale bar = 50 μm. Images shown are representative only of the quantification shown on right; quantification was determined from the entire cell layer. b Matrigel outgrowth (nubbin) assay to assess invasive potential. c Sulforhodamine B assay (n = 3). Error bars represent SEM, and statistical significance was determined by two-way analysis of variance with Bonferroni’s posttest; * p < 0.001. (i) Low seeding density: 2500 cells/well; (ii) high seeding density: 5000 cells/well seeding density. ns Not statistically significant