Table 3.
Primers used in this study
| Primer name | Nucleotide sequence | |
|---|---|---|
| L32 | RT sequence | CAGAAAACGTGCACATGAGCTGC |
| Forward sequence (outer nested) | CAGGGTTCGTAGAAGATTCAAGGG | |
| Reverse sequence (outer nested) | CTTGGAGGAAACATTGTGAGCGATC | |
| Forward sequence (inner nested) | GATCTTGATGCCCAACATTGGTTATG | |
| Reverse sequence (inner nested) | GCACTTCCAGCTCCTTGACG | |
| Carbonic Anhydrase 9 (for hypoxia validation) | Random priming used | |
| Forward | CCTCAAGAACCCCAGAATAATGC | |
| Reverse | CCTCCATAGCGCCAATGACT | |
| E-cadherin | RT sequence | GTCAGCCAGCTTCTTGAAGCGATT |
| Forward sequence | GCCCTGCCAATCCCGATGAAA | |
| Reverse sequence | GGGGTCAGTATCAGCCGCT | |
| Vimentin | RT sequence | CCGTGAGGTCAGGCTTGGAAA |
| Forward sequence | GCTTCAGAGAGAGGAAGCCGAAAA | |
| Reverse sequence | CCGTGAGGTCAGGCTTGGAAA | |
| Endogenous E-cadherin (CDH1-3′UTR) | RT sequence | GCACTTGGGGATTCTGGGCTTT |
| Forward sequence | GTGCCTAAAGTGCTGCAGCCAAA | |
| Reverse sequence | GTACAAACCACGGATCTTGTGTCAGAAA | |
| Exogenous E-cadherin (for 468-CDH1 construct) | RT sequence | GAAAGGACAGTGGGAGTGGCACTTT |
| Forward sequence | CCTGAACTCCTCAGAGTCAGACAAA | |
| Reverse sequence | GTGGCACCTTCCAGGGTCAAGGAA | |
| E-cadherin shRNA (for 468-shCDH1 constructs) | RT sequence | CCAGCTCAGCCCGAGTGGAAAT |
| Forward sequence | CCTCCCATCAGCTGCCCAGAAAA | |
| Reverse sequence | CTCTGTCACCTTCAGCCATCCTGTTT | |
| Snail 1 | RT sequence | CGCAGACAGGCCAGCTCAGGAAT |
| Forward sequence | CACATCCTTCTCACTGCCATGGAATT | |
| Reverse sequence | GCTGCCCTCCCTCCACAGAAAT | |
| Estrogen Receptor 1 | RT sequence | CCAGGGCCACGCTGGGAAATGAA |
| Forward sequence | GTTCCAGTGGGCACTGTACTTGGATCTT | |
| Reverse sequence | CAGCTCCATGCCCCAGGGCTAAAT | |
Abbreviations: RT reverse transcription, shRNA Short hairpin RNA