Pericyte (PC) protein alignment in vitro. Alignment indices of PC proteins collagen IV (A), laminin (B), fibronectin (C), and collagen I (D) as determined by a fast Fourier transform. E–H: Representative processed images of collagen IV (E), laminin (F), fibronectin (G), and collagen I (H) used for protein alignment analysis. I: Representative images of eosin-stained protein samples used for pore-size quantification. Data are expressed as means ± SEM (A–D). ∗P < 0.05, ∗∗∗P < 0.001. Scale bars: 100 μm (E–H); 5 μm (I).