Table 2. Additional information for enzymatic assays of soils.

Standards for the phenol oxidase assay were created by reacting a known mass of l-3,4-dihydroxyphenylalanine substrate with a horseradish peroxidase.

Enzyme	Shorthand	Activity	Substrate	Standard	Target
α-Glucosidase	AG	Hydrolytic	4-MUB-α-d-glucopyranoside	4-MUB	Starch
β-Glucosidase	BG	Hydrolytic	4-MUB-β-d-glucopyranoside	4-MUB	Cellulose
N-Acetyl-β-glucosaminidase	NAG	Hydrolytic	4-MUB-N-acetyl-β-d-glucosaminide	4-MUB	Chitin
Phenol oxidase	POX	Oxidative	l-3,4-Dihydroxyphenylalanine	N/A	Lignin
Leucine aminopeptidase	LAP	Hydrolytic	l-Leucine-7-amido-4-methylcoumarin HCl	7-Amino-4-methylcoumarin	Protein

Note:

4-MUB, 4-methylumbelliferyl.