(a) Mannose-modified
HNPs were composed of NIPAm (63.5 mol %),
TBAm (28 mol %), AAc (5 mol %), BIS (2 mol %), and p-acrylamidophenyl-α-d-mannopyranoside (Man, 1.5 mol
%). The lectin concanavalin A (ConA, purple) binds specifically to
Man sugar units (green) in the mannose-modified HNPs. (b) Δ%RNP frequency distribution histograms obtained
from the SPRI adsorption measurements of mannose-modified HNPs alone
(transparent blue bars) and mannose-modified HNPs in the presence
of 500 nM ConA (solid red bars). Δ%RNP frequency distribution histograms are also plotted for additional
SPRI adsorption measurements of mixtures of mannose-modified HNPs
and 500 nM ConA in the presence of (c) 1 mM mannose (solid green bars)
and (d) 10 mM mannose (solid orange bars). The Δ%RNP frequency distribution histogram for mannose-modified
HNPs alone is replotted in (c) and (d) for comparison.