Fig. 3. Biological characterization of the carborane 1. (a) Cell proliferation in human MCF-7 breast adenocarcinoma cells. P < 0.01 vs. control cells. (b) Differentiation activity in human HaCaT keratinocytes cells. (c) Vitamin D receptor binding. Competitive binding of 1,25D and carborane 1 to the full-length human VDR. IC50 values are derived from dose–response curves. (d) Transcriptional responses of hVDR in HEK293 EBNA cells. (e) Real-time PCR of CYP24A1 mRNA levels in human MCF-7 breast cancer cells. (f) Calcium levels in sera of mice treated with 1,25D and carborane 1. Error bars represent standard deviation (SD).
