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. Author manuscript; available in PMC: 2017 Jul 27.
Published in final edited form as: Nat Commun. 2015 Apr 8;6:6749. doi: 10.1038/ncomms7749

Figure 6. OCT4 favours an enhancer/promoter loop within the Sox-17 locus and breaks a Sox-2 enhancer/promoter loop.

Figure 6

3C experiments were performed on digested and religated chromatin from undifferentiated ZHBTc6 cells or OCT4-induced mesendodermal cells. Primers spanning within the Sox-2 and Sox-17 loci and numbered as in Table 2 were used in PCR. Control primers such as in exons were used as controls (cartoons, a-c). The graphs show the frequency of interactions within Sox loci on chromosomes 1 (a) or 3 (b) in both control (ZHBTc6 non-induced cells) and Oct4OE (ZHBTc6 induced) mesendodermal cells. Interactions frequencies were normalized using the GAPDH locus whose 3D configuration was assumed not to change, as GAPDH is expressed to the same extent in both pluripotent ESCs and OCT4OE (ZHBTc6 induced) mesendodermal cells. The relative interaction frequency value is thus set to one for the interaction measured between the control GAPDH fragments. Data are representative of four experiments (mean ± s.e.m.).