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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Plast Reconstr Surg. 2017 Aug;140(2):316–326. doi: 10.1097/PRS.0000000000003542

Figure 4. Explanted fibroblasts maintain Thy1 expression.

Figure 4

Thy1 expression is maintained in fibroblasts following explant. A) Fibroblasts were fixed and stained for Thy1 (blue), filamentous actin (red), and nucleic acid (green) to confirm presence of Thy1. B) Western analysis shows that, while all fibroblasts expressed Thy1 protein, fibroblasts explanted from IR Capsule exhibited a 5 -fold increase in Thy1 compared to NIR Capsule. C) Finally, flow cytometry analysis demonstrates a positive shift in signal intensity in Thy1 stained cells compared to unstained fibroblasts. Thy1 protein normalized to β-tubulin. * = p<0.05.