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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Plast Reconstr Surg. 2017 Aug;140(2):316–326. doi: 10.1097/PRS.0000000000003542

Figure 5. Thy1 depletion reduces αSMA and collagen.

Figure 5

A) Thy1 protein knockdown achieved via short hairpin RNA coding lentiviral vector (shThy1) also reduces αSMA and collagen compared to control shRNA lentiviral vector (Control). Thy1 and αSMA normalized to β-tubulin. Collagen quantification based on equal volume media loading. B) Representative Western blot showing Thy1 and αSMA normalized to β-tubulin. Collagen quantified via slot blot based on loading equal culture supernatant volume. ** = p<0.01; **** = p<0.0001.