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Qualitative changes observed in shThy1 fibroblasts (A&C) compared to control fibroblasts (B&C) include elongated soma with fewer cytoplasmic projections, rounded shape, and greater in vitro cell density. Cells were stained for DNA (green) using Sytox Green, filamentous actin (red) using phallodin-AF594, and Thy1 (blue) using anti-Thy1 antibody conjugated to BV421.
