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. 2017 Aug;199(2):120–131. doi: 10.1016/j.jsb.2017.05.013

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© 2017 The Authors. Published by Elsevier Inc.

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 4 — Comparison of WF to SR highlights significantly improved lateral resolution: GFP-C1. a: WF image showing localisation of the GFP-C1 construct (and hence the lipid DAG) within a HeLa cell, and SR reconstruction from a sequence of approximately 30,000 images, representing 71,413 individual localisations. Enlargements of the boxed areas show the improved precision of GFP-C1 localisation from SR based image reconstruction. b: Micrograph showing the BSE signal from an individual cell expressing the GFP-C1 construct with WF image and SR reconstruction overlaid. c, d: Enlargements of the boxed areas in B showing localisation of the GFP signal to membranes. The improved lateral resolution of the SR-EM overlay reveals DAG within Golgi stacks, endoplasmic reticulum (arrows), and a putative autophagosome (asterisk). G: Golgi, M: mitochondria, N: nucleus. Scale bars - 5 µm (a, b), 1 µm (A inset, c, d).