Fig. 5.
The quality of SR image reconstructions is directly influenced by the vacuum pressure at the time of acquisition. a: WF, SR and SEM images showing a cropped area of the GFP-C1 expressing cell shown in Fig. 4, acquired with the chamber at atmospheric pressure. The reconstruction represents ∼ 6,000 localisations. b: An area matching that shown in A from the preceding section in the series, acquired at a chamber pressure of 200 Pa, maintained at partial pressure using water vapour. The SR reconstruction represents ∼25,000 localisations. c: An area matching that shown in A and B from the next section in the series, acquired with the chamber pumped to high vacuum (10−3 Pa). The SR reconstruction represents ∼4,000 localisations. G: Golgi, M: mitochondria. Asterisk: putative autophagosome. Scale bar – 2 µm.