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. 2017 Jul 27;8:176. doi: 10.1186/s13287-017-0628-9

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Internalization of HLSC-EVs in hepatocytes differentiated from ASS1-HLSCs. Representative confocal microscopy showing the internalization of 1 × 10¹⁰ Dil-labeled EVs in hepatocytes differentiated from ASS1-HLSCs after 6 h of incubation at 37 °C. Z stack analysis shows the presence of EVs within the cytoplasm of the cells indicating an effective uptake of vesicles; scale bar = 50 μm. Data represent one of three experiments performed with similar results