Figure 9. Detection of α 11.2 distal C-terminus.
CNC2 immunoblots showed an immunoreactive band migrating near 30 kDa in Triton X-100 forebrain extracts after immunoprecipitation with FP1. Signal was absent in immunoprecipitates from cKO samples. Gels were polymerized from 13% acrylamide. Note that this interaction was variable, with roughly half of 8 IPs tested showing a clear, specific band, perhaps because this fragment is unstable and proteolytically degraded. Also, the 50 kDa bands are most likely the heavy chains of the immunoprecipitating antibody.