Figure 8. The emergence of memory CD8 ⁺ Teffs in cell cycle and with mucosal homing capacity post-norovirus infection.

The percentage change in frequency of Ki-67 ⁺ CD8 ⁺ cmTeffs ( A) and Ki-67 ⁺ CD8 ⁺ emTeffs ( B). Contour plots for each cell subset show Ki-67 and CD25 expression pre-IL-2 administration and at the peak of the response in the norovirus-infected participant. Timecourse analysis of the frequency of CD103 ⁺CD45RA ⁻CD27 ⁻ CD8 ⁺ emTeffs of total CD8 ⁺ T cells ( C). Contour plots showing the frequency of CD103 ⁺β7integrin ^−/loCD45RA ⁻CD27 ⁻ CD8 ⁺ emTeffs at day 0 and day 7 post-IL-2 ( D). ( E) The change in frequency of CD103 ⁺β7integrin ⁺CD45RA ⁻CD27 ⁺ CD8 ⁺ cmTeffs of total CD8 ⁺ T cells relative to baseline levels. ( F) Contour plots showing the frequency of CD103 ⁺β7 integrin ⁺CD45RA ⁻CD27 ⁺ CD8 ⁺ cmTeffs at day 0 and day 7 post-IL-2. ( A, B, C and E) data were normalised to and expressed as percentage change from baseline (day 0) in uninfected participants (filled squares +/- SEM) or the norovirus-infected participant (filled circles). Analyses were performed using cryopreserved PBMCs. The shaded area indicates the period of reported gastroenteritis.