Reagent	Volume (μl)
10x High Fidelity buffer	5
50 mM MgSO4	2
10 mM dNTP	1
10 μM forward sgRNA primer	2
10 μM reverse common sgRNA	2
DNA (20 ng/μl)	2
Platinum® Taq DNA polymerase HF	0.2
dH2O	35.8
Total volume	50