Fig. 3.

2D Cell Monolayers in Microfabricated Geometries. (A) Microscale glass wires embedded in PDMS, (B) Individual cells can detach and scatter along glass wires from multicellular groups. Reproduced from⁸⁷ with permission from the National Academy of Sciences. (C) Microcontact printing using PDMS stamps to pattern fibronectin shapes on soft PA gels. (D) Cell monolayers display stem-like markers at the periphery and corners of these shapes. Reproduced from⁸⁸ with permission from Nature Publishing Group. (E) Cells undergoing confined migration within arrays of microscale PDMS pillars, with a glass ceiling. (F) Cells transition from collective to individual migration after EMT. Reproduced from⁸⁹ with permission from Nature Publishing Group.