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Assignment of microcrystalline (MPD-ub) G53A ubiquitin. (A) 2D H-N correlation spectrum recorded with a 1H detected hNH experiment based on cross-polarization steps. (B) Example assignment strips from 3D hCANH, hcoCAcoNH and hcaCBcaNH spectra. The residues shown in (B) are indicated in the 2D spectrum in (A).
