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. Author manuscript; available in PMC: 2017 Nov 20.
Published in final edited form as: Solid State Nucl Magn Reson. 2017 Apr 14;87:86–95. doi: 10.1016/j.ssnmr.2017.04.002

Figure 8.

Figure 8

Amide proton R1ρ relaxation dispersion data in wild-type ubiquitin microcrystals (MPD-ub), obtained at a MAS frequency of 44.053 kHz and 600 MHz 1H Larmor frequency. The protein was reprotonated at exchangeable sites at a level of ~35%. The top panel show spin-lock RF-field dependencies for four residues. The lower two panels show 1H R1ρ rate constants as a function of residue number obtained at 10 kHz spin-lock RF field strength (top) and the difference of 1H R1ρ obtained at RF field strengths of 42 and 10 kHz (bottom). Equivalent data recorded at 800 MHz Larmor frequency and 35 kHz MAS are shown in Figure S5, and reveal very similar behavior, in particular with respect to the first loop and the β-turn region.