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. 2016 Jan 1;37(7):2555–2566. doi: 10.1177/0271678X16671147

Figure 4.

Figure 4.

Role of vitamin D3 (VitD3) in upregulation of endogenous osteopontin (OPN) expression after subarachnoid hemorrhage (SAH). (a) Pre- and Post-treatment of VitD3 increased VDR brain expression. Pre-SAH + VitD3, intranasal treatment 30 ng/kg/day VitD3 24 h before SAH; Post-SAH + VitD3, intranasal treatment 30 ng/kg/day VitD3 1 h after SAH. *P < 0.05 and †P = 0.057 vs. sham; n = 5 per group. (b) Pre- and Post-treatment of VitD3 increased OPN cerebral expression. *P < 0.05 and **P < 0.01 vs. sham; #P < 0.05 vs. SAH. n = 5–6 per group. (c) Immunohistochemical staining of OPN (green) and astrocytes (GFAP, red). Nuclei are stained with DAPI (blue). Top panel indicates the location of immunohistochemical staining (small black box). Arrows indicate colocalization of OPN and GFAP. n = 3 per group. Bar = 50 µm.