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. 2017 Mar 24;37(7):2598–2613. doi: 10.1177/0271678X17700665

Figure 1.

Figure 1.

BBB in vitro model. (a) Graph showing TEER values measured from pBECs grown in non-contact co-culture with primary rat astrocytes. Measurement was done at 24 h intervals following incubation with differentiation factors using EndOhm 12 device. Data corrected for values of TEER measured on empty Transwell, n = 11, mean ± SD and (b) immunostaining of pBECs grown in non-contact co-culture with primary rat astrocytes for proteins associated with TJs. Cells fixed and immunostained on third day of culture; nucleus (blue), TJ markers (red and green), scale bar 10 μm.