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. 2017 Mar 24;37(7):2598–2613. doi: 10.1177/0271678X17700665

Figure 5.

Figure 5.

Detection of MPR300 trafficked across cells in pBECs. (a) Scheme showing concept of experiment and experimental set-ups used in study. Four combinations were prepared (Transwell/well): (I) Rabbit anti-MPR300 (1:100)/Donkey anti-rabbit IgG conjugated to AlexaFluor 488 (1:200); (II) Donkey anti-rabbit IgG conjugated to AlexaFluor 488/Rabbit anti-MPR300; (III) Non-treated (NT)/donkey anti-rabbit IgG conjugated to AlexaFluor 488 and (IV) Donkey anti-rabbit IgG conjugated to AlexaFluor 488/non-treated (NT) and (b) Immunostaining of pBECs for trafficked across cell plasma membranes MPR300 (green) and Vps35 (red); nucleus (blue; in all pictures); scale bar 10 μm; Z-stack of pBECs presented as maximum projection over Z; marked region presented in inset for separate channels, from left: receptor staining, marker staining, merged.