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. 2017 Jul 17;127(8):3126–3135. doi: 10.1172/JCI92684

Figure 2. Ex vivo modeling of serial VOR exposures.

Figure 2

(A) Schematic of ex vivo dosing experiment. Resting CD4+ T cells from aviremic participants were exposed to media or 335 nM VOR (or PHA as a comparative control) for 6 hours, rca-HIV RNA was measured in an aliquot (B), and the remaining cells were washed and placed in culture. Cells then received no VOR or a second 6-hour pulse of VOR at 18 hours, 42 hours, or 66 hours following the first pulse to model daily, every-2-day, or every-3-day dosing regimens. rca-HIV RNA was measured in cells that had never been exposed to VOR (circles), were pulsed once (squares), or pulsed twice (triangles) at 24 hours (C), 48 hours (D), and 72 hours (E). Each data point represents 1 million resting CD4+ T cells. n = 3 independent experiments. Data represent the mean ± SD. *P < 0.05,**P < 0.005, and ***P < 0.0001, by Mann-Whitney U test.