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. 2017 Jul 27;12(7):e0181864. doi: 10.1371/journal.pone.0181864

Fig 7. Hemolytic activities of Igl1-attenuated Entamoeba histolytica trophozoites.

Fig 7

Vector transfected or Igl1 gene-silenced E. histolytica trophozoites (1×105) were incubated with HoRBCs at 37°C for 1 h. A and C. Images of HoRBCs in a 96-well plate just after mixing with trophozoites (0 hr) and after incubation for 1 h with trophozoites (1 hr). B and D. Released hemoglobin (Hb) concentration in the supernatant of a mixture of trophozoites and HoRBCs after incubation for 1 h at 37°C. Data are the mean ± SD from 5 independent experiments. **p< 0.01 by ANOVA with Dunn test. Control: vector transfected E. histolytica trophozoite, gsIgl1A and gsIgl1B: Igl1 gene-silenced E. histolytica trophozoite.