Table 1. Oligonucleotide primers used in the study.

Primer	Positiona	Sequence (5′ to 3′)b	Ref.
(for Eh Igls)
EhIgl-S14	40–59	CCCTCGAGGATTATACTGCTGATAAGCT	[9, 12]
EhIgl2-S14	40–70	CCCTCGAGGATTATACTGCTGATAAACTCATTAATAACC	[7]
EhIgl-S294	880–898	CCCTCGAGACAGAAGAAAATAAATGTA	[9, 12]
EhIgl-S603	1807–1827	CCCTCGAGGAAGGACCAAATGCAGAAGAT	[9, 12]
EhIgl-AS753	2244–2259	CCCTCGAGTTATAGCCTTTGTTCAGTG	[9, 12]
EhIgl-AS1088c	3247–3264	CCCTCGAGTTAAATGCCTTTAGCTCCATT	[9, 12]
(for Ed Igls)
EdIgl-S14	40–59	CCCTCGAGGAGTACAAAGCTGATAAACT	[8]
EdIgl2-S14	40–63	CCCTCGAGGATTACAAAGCTGATAAACTCATC	[8]
EdIgl-S604	1810–1830	CCCTCGAGGAAGGACCAAATGAAGAAGAT	*
EdIgl-AS1097c	3274–3291	CCCTCGAGTTAAATTCCTTTACTTCCATT	[8]

^a Nucleic acid numbering is based on the E. histolytica Igl1 and Igl2 gene sequences (AF337950 and XM_647302) and E. dispar Igl1 and Igl2 gene sequences (AB287423 and AB287424).

^b Nucleotides added for cloning and translation termination are underlined.

^c EhIgl-AS1088 and EdIgl-AS1097 are common for EhIgl2 and EdIgl2 respectively.

* This study.