Fig 5. Effect of miR-16-5p and miR-30d-5p on cell growth and average cell diameter, respectively compared to scramble control.

HASM cells were transfected with 10 nM of either scramble control or miR-16-5p mimic (left panel; or miR-30d-5p in right panel). Seventy-two hours after transfection, cells were trypsinized and measured for both cell number and cell size by Moxi Z Cell Analyzer. Cell growth was presented as the percentage of cell number relative to the scramble control. Average cell size (um in diameter) was compared in mimic-transfected versus scramble-transfected HASM cells. Data were obtained from three independent experiments. For the miR-16 and miR-30d experiments, the p-value is 0.0009 and 0.03, respectively.