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. 2017 Jul 27;12(7):e0182057. doi: 10.1371/journal.pone.0182057

Fig 3. Influence of GW-A2 on the prophylactic and therapeutic effects of LPS-induced NO generation.

Fig 3

(A) RAW 264.7 macrophages were incubated for 0–24 h with or without GW-A2, followed by 24 h with or without 0.1 μg/ml of E. coli LPS. The levels of NO in the culture medium were measured by the Griess reaction. (B) RAW 264.7 macrophages were incubated for 24 h with or without 0.1 μg/ml of E. coli LPS, in the presence or absence of 2 μM of GW-A2 in the last 0–24 h. The levels of NO in the culture medium were measured by the Griess reaction. (C) RAW 264.7 macrophages were incubated for 0–24 h with or without GW-A2. Then, the cells were washed and the supernatant was replaced with fresh media and stimulated for 24 h with or without 0.1 μg/ml of E. coli LPS. The levels of NO in the culture medium were measured by the Griess reaction. The data are expressed as the mean ± SD of three independent experiments. *, ** and *** indicate significant differences, representing p < 0.05, p < 0.01 and p < 0.001, respectively compared to LPS alone.