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. Author manuscript; available in PMC: 2018 Jul 6.
Published in final edited form as: Cell Stem Cell. 2017 Jun 22;21(1):51–64.e6. doi: 10.1016/j.stem.2017.05.020

Figure 5.

Figure 5

HIOs and HCOs maintained regional identity following transplantation in vivo.

(A–E) H&E staining of biopsies from human jejunum and colon and of NOGGIN-derived HIOs, control HIOs, and BMP2-derived HCOs that were transplanted underneath the mouse kidney capsule and grown for 8–10 weeks in vivo. The samples of the same conditions were stained with the proximal intestinal marker GATA4 (F–J), the distal intestinal marker SATB2 (K–O), the Paneth cell marker DEFA5 (P–T), and the colon-specific goblet cell marker MUC5B (U–Y). Note that although GATA4 and SATB2 double staining was done in different channels but on the same slides for panels (F–O), they are shown as individual pseudocolored (red) images. For human biopsies n=2. For transplanted NOGGIN treated organoids n=12, for control organoids n=7, and for BMP2 treated organoids n=16. Scale bars= 50 μm.