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. Author manuscript; available in PMC: 2018 Jul 6.
Published in final edited form as: Cell Stem Cell. 2017 Jun 22;21(1):51–64.e6. doi: 10.1016/j.stem.2017.05.020

Figure 6.

Figure 6

In vivo grown organoids express region specific hormones.

Analysis of expression of the regionally expressed hormones (A–D) Ghrelin (GHRL), Motilin (MLN), (E–H) GIP, (I–L) GLP-1, (M–P) PYY and (Q–T) INSL5 in HIOs and HCOs grown for 8–10 weeks underneath the mouse kidney capsule. The proximally enriched hormones GHRL, GIP and MLN were enriched in NOGGIN and control HIOs (A–H). The distally enriched hormones GLP-1 and PYY were enriched in BMP2-derived HCOs (I–O). The colon specific hormone INSL5 was only present in HCOs (Q–T). Data is representative of at least 5 transplanted organoids per condition. Insets in (A) and (B) show GHRL and MLN double positive cells. (D, H, L, P, T) FPKM values for GHRL, MLN, GIP, GLP1, PYY, and INSL5 are from RNA-seq data. FPKM values represent 3 biological replicates per condition. Scale bars= 30 μm.