Fig. 1.

Loss of melanoregulin is associated with LC3 accumulation. a Diurnal expression profile of proteins associated with hybrid autophagy–phagocytosis degradation processes. RPE cell lysates were prepared from 6-month-old Mreg ^dsu/dsu and Mreg^+/+ mice at the times indicated after light onset (t=0) and immunoblotted for proteins indicated. A representative immunoblot is shown for each time point. b Quantitation of Mreg ^dsu/dsu and Mreg^+/+ mouse RPE immunoblots. All blots were normalized to β-actin as a loading control. Results are average of three independent RPE lysate samples, each sample preparation consisting of six mouse eyes. Error bars indicate ±SEM, (*p<0.01 or **p<0.005, n=18)