Figure 3.

Sirt3 overexpression attenuates ROS production in oocyte. Exogenous Myc-Sirt3 mRNA or PBS was microinjected into fully-grown GV oocytes from diabetic mice, which were arrested at GV stage in M16 medium containing 2.5 μΜ milrinone for 20 hours to allow synthesis of new Myc-Sirt3 protein. Following in vitro-maturation, MII oocytes were stained by CM-H2DCFAD (green) to evaluate ROS levels. (A) Representative images of CM-H2DCFAD fluorescence in control, diabetic, diabetic+PBS and diabetic+Sirt3 oocytes. Scale bar: 25 μm. (B) Quantitative analysis of fluorescence intensity shown in B (n = 10 oocytes for each group). Data were expressed as mean ± SD from 3 independent experiments. *p < 0.05 vs control.