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. 2017 Jun 29;16(13):1302–1308. doi: 10.1080/15384101.2017.1320004

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Figure 4. — Sirt3 overexpression lowers the acetylation levels of SOD2K68 in oocytes from diabetic mice. Control (n = 77), diabetic (n = 71), diabetic+PBS (n = 80) and diabetic+Sirt3 (n = 76) MII oocytes were labeled with acetyl-SOD2K68 antibody and counterstained with Hoechst 33342 for chromosomes. (A) Representative images show the AcSOD2K68 signal and DNA in oocytes. Scale bar: 25 μm. (B) Quantitative analysis of fluorescence intensity shown in A. Data were expressed as mean ± SD from 3 independent experiments. *p < 0.05 vs control.