Figure 5.

SOD2K68R partly prevents oxidative stress in oocytes from diabetic mice. SOD2 WT or SOD2K68R mutant mRNA was microinjected into fully-grown GV oocytes from diabetic mice, which were arrested at GV stage in M16 medium containing 2.5 μΜ milrinone for 20 hours to allow synthesis of new protein. Following in vitro-maturation, MII oocytes were stained with CM-H2DCFAD (green) to evaluate ROS levels. (A) Representative images of CM-H2DCFAD fluorescence in control, diabetic, diabetic+SOD2 and diabetic+SOD2 (K68R) oocytes. Scale bar: 25 μm. (B) Quantitative analysis of fluorescence intensity shown in A (n = 10 oocytes for each group). Data were expressed as mean ± SD from 3 independent experiments. *p < 0.05 vs control.