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. 2017 Jul 27;12(7):e0182141. doi: 10.1371/journal.pone.0182141

Fig 2. Knockdown of ERRγ attenuates ACEA-mediated induction of fibrinogen.

Fig 2

(A) ACEA-mediated induction of fibrinogen expression. Huh7 cells were treated with ACEA (10 μM) for the indicated time periods. Total RNAs were extracted for qPCR analyses. (B-C) Huh7 cells were treated with ACEA (10 μM) for 24 h. Total protein was extracted for western blotting (B). Cell culture media were collected to determine fibrinogen levels (C). * p<0.05, ** p<0.01. All data are representative of at least three independent experiments. Error bars show SEM. (D) Huh7 cells were treatment with ACEA in the continued presence or absence of AM251 for 24 h. qPCR were performed to measure mRNA levels. * p<0.05. ** p<0.01. (E-G) qPCR (E) and western blot (F) analysis showing mRNA and protein levels of ERRγ, FGA, FGB, and FGG in Huh7 cells. Huh7 cells were infected with Ad-Usi or Ad-shERRγ for 48 h, followed by treatment with ACEA (10 μM). Cell culture media were collected to determine fibrinogen levels (G).Western blot images were cropped with a black cropping line. All gels for Western blot analysis were run under the same experimental conditions. Full uncropped blots are available as S1 and S2 Figs.