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. 2017 Jul 27;12(7):e0180881. doi: 10.1371/journal.pone.0180881

Fig 5. RelB expression in increased in lung fibroblasts in response to IL-1β.

Fig 5

Human lung fibroblasts were cultured with 10 ng/ml of rhIL-1β for 6 or 24 hours and RelB mRNA assessed by qRT-PCR and cell lysate for detection of RelB protein by western blot. (A) RelB mRNA- There was a significant increase in RelB mRNA in lung fibroblasts derived from Normal, At Risk and COPD subjects compared to respective unstimulated controls (*** p < 0.001). Results are expressed as the mean ± SEM of three strains from each subject group. (B) RelB protein- There was a noticeable and significant increase in RelB protein expression upon stimulation of lung fibroblasts with IL-1β for 24 hours (*p < 0.05 for each fibroblast group compared to their respective unstimulated controls). There was no difference in RelB induction between the three groups. Results are expressed as the mean ± SEM of three independent experiments.