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. 2017 Aug 1;28(16):2190–2201. doi: 10.1091/mbc.E17-05-0278

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© 2017 Meng et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — pY14Cav1 shifts the vinculin tension distribution of focal adhesions. (A) FRET AB measurements of vinculin tension of focal adhesions of PC3 cells treated with Jasp and LatA were binned at interval of 0.04 unit. Focal adhesions were arbitrarily divided into groups of high (FRET 0–0.12), medium (FRET 0.12–0.24), and low (FRET 0.24 – 0.8) tension by comparing the Jasp- and LatA-treated cells. (B–D) Percentage of low-/medium-/high-tension focal adhesions in PC3 and DU145 cells with various treatments and transfections as noted. Data represent mean ± SEM of three independent experiments (n > 20 for each cell type/treatment for each experiment). One-way ANOVA with Tukey posttest; *p < 0.05; **p < 0.01; ***p < 0.001.