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. 1982;1(10):1149–1153. doi: 10.1002/j.1460-2075.1982.tb00005.x

Visualization of Z sequences in form V of pBR322 by immuno-electron microscopy.

M C Lang, B Malfoy, A M Freund, M Daune, M Leng
PMCID: PMC553181  PMID: 7188247

Abstract

Form V DNA has been prepared from pBR322 DNA by annealing covalently closed complementary single strands. Specific rabbit antibodies to Z-DNA were shown by radioimmunoassay and electron microscopy to react with form V DNA of pBR322. The bound antibodies were visualized either directly (on synthetic polynucleotides in Z-form), or after reaction with goat anti-rabbit immunoglobulin labeled with ferritin (on form V DNA).

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Selected References

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