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The W303-1A- and YJM789-derived chromatids are shown in red and blue, respectively. On the W303-1A-derived homolog, the HS4 hotspot is flanked by the hphMX4 and URA3 markers; SUP4-o is located near the telomere of the YJM789-derived homolog. Since the recombinogenic DSB is located at HS4, HS4-initiated events usually result in loss of HS4 as shown.
