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. 2017 Jun 16;6:e25421. doi: 10.7554/eLife.25421

Figure 1. AOB MCs express robust, slowly emerging self-inhibition.

(A) Schematic of dendrodendritic self-inhibition pathway in MCs. (B) Left, dye-filled MC imaged after recording. S, soma; d, dendritic tufts that integrate sensory inputs; p, recording pipette. Right, brief, high-frequency spike trains trigger modest self-inhibition (gray, standard ACSF; black, after blocking inhibition with 15 µM BMI; IPSP, inhibitory postsynaptic potential). (C) Pharmacologically isolated self-inhibition in AOB MCs. Colored traces show individual cells; black trace, average; mean Vinh = −1.4 ± 0.27 mV (n = 8 cells in 5 mice). (D,E) Prolonged firing elicits robust MC self-inhibition in AOB but not MOB (black and purple respectively). Boxes show expanded view of barrages of IPSPs in AOB MCs, indicated by arrowheads, which only emerge after several seconds of firing. (F) Self-inhibition contributes to stronger decay of MC firing rates in AOB during extended stimuli (n = 9 and 9 cells in 5 and 5 mice for AOB and MOB respectively). (G) Initial and final firing rates during MC spike trains in MOB and AOB (purple and gray respectively).

DOI: http://dx.doi.org/10.7554/eLife.25421.003

Figure 1—source data 1. This spreadsheet contains the initial and final firing rates for the individual neurons shown in Figure 1G.
These data can be opened with Microsoft Excel or with open-source alternatives such as OpenOffice.
DOI: http://dx.doi.org/10.7554/eLife.25421.004
elife-25421-fig1-data1.xlsx (32.8KB, xlsx)
DOI: 10.7554/eLife.25421.004

Figure 1.

Figure 1—figure supplement 1. Robust self-inhibition regulates spiking of AOB MCs.

Figure 1—figure supplement 1.

(A) Eliminating self-inhibition by blocking fast synaptic transmission increased the overall firing rate of MCs. (B) Blocking synaptic inhibition also eliminated the barrages of IPSPs that emerged later in train and persisted after the offset of spiking. (C) Firing of AOB MCs is consistently enhanced after blocking inhibition (p=0.06; t-test; n = 6 MCs in 6 mice).
DOI: http://dx.doi.org/10.7554/eLife.25421.005